Distribuição de estresse em incisivo central superior sob situações traumáticas semelhantes com diferentes cargas: uma análise tridimensional de elementos finitos / Stress distribuition on maxillary central incisor under similar traumatic situations with different loading forces: a 3-D finite element analysis

Aim: The present study aimed to analyze the stress distribution in the dentoalveolar structures of a maxillary central incisor submitted to two situations of impact loading. Materials and Methods: The following loading forces were applied using a three-dimensional finite element model: a force of 500 N at an angle of 45° on the buccal surface of the crown and a 2000 N force acting in the same direction and surface of the tooth. Results: Harmful stress was observed in the second situation, suggesting damage to both the tooth and adjacent tissue. However, the damage found in soft tissues such as dental pulp, was negligible. Conclusion: Injuries resulting from the traumatic situations were more damaging to the integrity of the tooth and its associated hard-tissue structures.

Concepções de promoção da saúde presentes nas Conferências Nacionais de Saúde Bucal / Conceptions of health promotion present at the National Oral Health Conferences

Objetivos: Analisar as Conferências Nacionais de Saúde Bucal, com ênfase na concepção e fundamentos da promoção da saúde, entendendo este recorte como um alinhamento do papel social da Odontologia. Métodos: Trata-se de um estudo descritivo de cunho documental, realizado a partir da análise dos relatórios finais das três conferências nacionais de saúde bucal disponíveis no site do Ministério da Saúde. Para fins de análise desta pesquisa, de base documental, utilizou-se o referencial da análise temática para apreender as concepções e os fundamentos de promoção da saúde presentes. Resultados: Pôde-se observar que a odontologia foi gradativamente incorporando o sentido e o significado da promoção da saúde enquanto referencial orientador para ações de saúde bucal. Evidenciou-se que a visão ampliada de saúde faz parte do relatório das três conferências, e que concepções de promoção da saúde concernentes com o cenário internacional da época foram assumidas, mesmo ainda evidenciando-se ações preventivas de caráter vertical. Fundamentos importantes para a promoção da saúde também estão presentes, tais como intersetorialidade, integralidade das ações, equidade, autonomia e participação popular. Conclusões: Tais concepções foram de fundamental importância para agregação junto a uma Política Nacional de Saúde Bucal que viria a se concretizar anos depois.

Objective: To analyze the National Oral Health Conferences, with emphasis on design and foundations of health promotion, as an alignment of the social role played by dentistry. Methods: It is a descriptive study, conducted with basis on the documentary analysis of the final reports of the three national conferences of oral health, available on the website of the Ministry of Health. The analysis in this evidence-based research was inspired by the reference of thematic analysis to grasp the existing concepts and foundations of health promotion. Results: It was observed that dentistry was gradually incorporating the sense and meaning of health promotion as a guiding reference for oral health strategies. It was evident that the broader view of health is part of the reports of the three conferences and that concepts of health promotion pertaining to the international scene at the time were taken even further, evidencing preventive actions of vertical character. Relevant basis for health promotion are also present, such as intersectionality, integrality of actions, equity, autonomy and popular participation. Conclusion: Such views have been of great importance for aggregation along a national oral health policy that would become a reality, years later.

Antifungal activity of lectins against yeast of vaginal secretion

Lectins are carbohydrate-binding proteins of non-imune origin. This group of proteins is distributed widely in nature and they have been found in viruses, microorganisms, plants and animals. Lectins of plants have been isolated and characterized according to their chemical, physical-chemical, structural and biological properties. Among their biological activities, we can stress its fungicidal action. It has been previously described the effect of the lectins Dviol, DRL, ConBr and LSL obtained from the seeds of leguminous plants on the growth of yeasts isolated from vaginal secretions. In the present work the experiments were carried out in microtiter plates and the results interpreted by both methods: visual observations and a microplate reader at 530nm. The lectin concentrations varied from 0.5 to 256µg/mL, and the inoculum was established between 65-70% of trammitance. All yeast samples isolated from vaginal secretion were evaluated taxonomically, where were observed macroscopic and microscopic characteristics to each species. The LSL lectin did not demonstrate any antifungal activity to any isolate studied. The other lectins DRL, ConBr and DvioL, showed antifungal potential against yeast isolated from vaginal secretion. These findings offering offer a promising field of investigation to develop new therapeutic strategies against vaginal yeast infections, collaborating to improve women's health.

Perfil de proteases de lesões cutâneas experimentais em camundongos tratadas com a lectina isolada das sementes de Canavalia brasiliensis / Proteases profile of skin wounds treated with lectin from Canavalia brasiliensis seeds

O objetivo deste estudo foi determinar o perfil de proteases em lesões cutâneas experimentais tratadas com a lectina isolada das sementes da Canavalia brasiliensis (ConBr) livre e conjugada com o seu açúcar específico. Lesões cirúrgicas foram produzidas assepticamente na região dorsal de camundongos (n=120), divididos de acordo com o tratamento empregado: Grupo NaCl (NaCl 150mM), Grupo manose (manose 100mM), Grupo ConBr (ConBr 100µg mL-1) e Grupo ConBr/manose (solução contendo ConBr 100µg mL-1 preparada em manose 100mM). Amostras da área lesada foram coletadas para determinação do perfil de proteases e atividade colagenolítica no 2°, no 7° e no 12° dia de pós-operatório. O perfil das proteínas realizado através de eletroforese SDS-PAGE demonstrou a presença de proteínas com massa molecular de 67kDa em todos os grupos. O Grupo ConBr/manose apresentou a maior atividade colagenolítica no 12° dia de pós-operatório. A lectina isolada das sementes da Canavalia brasiliensis influenciou a expressão de proteases com atividade colagenolítica podendo assim interferir no processo cicatricial das lesões cutâneas em camundongos.

The objective of the present study was determining the proteases profile of cutaneous healings treated with free and conjugated lectin of Canavalia brasiliensis (ConBr) and their specific sugar. An aseptic wound was produced in the thoracic area of the mice (n=120), divided according to the employed treatment: NaCl Group (150mM NaCl), manose Group (100mM manose), ConBr Group (100µg mL-1 ConBr) and ConBr/manose Group (solution containing 100µg mL-1 ConBr prepared in 100mM manose). Samples of the injured area were collected for determination of proteases profile and collagenolytic activity on 2nd, 7th e 12th days after the surgery. Electrophoresis SDS-PAGE demonstrated proteins with molecular mass of 67kDa in all groups. Group IV presented the highest collagenolytic activity on the 12th day post surgery. ConBr lectin influenced proteases expression with collagenolytic activity thus being able to intervene on skin wound healing in mice.